ALK阳性的大B细胞淋巴瘤的免疫表型特点为EMA和浆细胞分化抗原CD138、VS38强阳性，CD3、CD20、CD79a、PAX-5通常阴性。大部分肿瘤细胞表达伴轻链限制性胞质型Ig（通常为IgA）。CD30通常阴性，部分病例局灶弱阳性。CD4、CD43、CD57、MUM1和CD45不同程度阳性，其异常表达T细胞（CD4）和（或）NK细胞（CD57）标记原因尚不清楚。穿孔素可局灶或强阳性，而迄今尚未发现穿孔素在其他类型B细胞淋巴瘤中弥漫强阳性表达的报道。可表达B细胞转录因子BOB.1和Oct-2。多组研究还显示无论免疫组织化学检测LMP-1还是EBER原位杂交检测EBV编码的mRNA，ALK阳性的大B细胞淋巴瘤均未发现EB病毒感染的证据。此外，肿瘤细胞具有很高的增殖活性^。

    ^{The frequency of expression of the various immunophenotypic markers is as follows: CD45,71%; CD20,3%(weak and focal); CD79a,16%; EMA,100%; CD30,6%(weak and focal); kappa or lambda light chain, 90%(most often IgA); CD138, 100%;CD4, 64%; and CD57, 40%. By definition, this lymphoma expresses ALK, with staining frequently confined to the cytoplasm, usually in a granular pattern. Cytokeratin exression has rarely been described, adding to the possible confusion with metastatic carcinoma, especially in combination with EMA positivity.(《Hematopathology》）}