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- 无知者求助
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We use paraffin embedded tissue block and H&E stain for our bone marrow biopsy. The slides are good for all the routine examinations. The paraffin embedded tissue section can be used for all the special stains including immunohistochemical study. I used the plastic embedded tissue processor many years ago. The only good thing is the plastic embedding can give you very thin sections, about 2-3 micro thick. But it takes very long time to process and can not be used for any imunostain. Not cost-effective.
| 以下是引用侯军在2010-5-8 10:53:00的发言： We use paraffin embedded tissue block and H&E stain for our bone marrow biopsy. The slides are good for all the routine examinations. The paraffin embedded tissue section can be used for all the special stains including immunohistochemical study. I used the plastic embedded tissue processor many years ago. The only good thing is the plastic embedding can give you very thin sections, about 2-3 micro thick. But it takes very long time to process and can not be used for any imunostain. Not cost-effective. |
对骨髓活检我们采用石蜡包埋组织块和HE染色。切片对所有常规检查都是比较好的。石蜡包埋组织切片可以被用于任何特染，包括免疫组化。许多年前我使用过树脂包埋处理仪器。用树脂包埋的唯一好处就是能够制作非常薄的切片，大约２－３微米厚。但是需要非常长的时间来处理，且不能用于免疫组化，不是物美价廉。
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本帖最后由 于 2010-05-16 11:08:00 编辑
Hi Everyone,
The following is our protocol for Bone marrow biopsy. It is relatively simple and very useful. I am happy with the morphology.
Important Points: 1) fix the core at least one hour, 2) use fast decal, 3) decal 20-30 minutes until it is flexible. determine your own time, 4) getting use to the morphology.
Let me know if any one want to try and any problems. Good luck !!!
Bone Marrow (Drexel University College of Medicine)
Procedure and Gross Description:
Specimen (Bone marrow biopsy)
is received in 10% buffered formalin (minimal fixation time: one hour ) with __ (number) of unstained slides. Describe grossly number, appearance (color,
consistency, uniformity), shape, and size (length and diameter). Put biopsy in
a cassette, which is labeled with surgical accession number, patient’s
initials, and cassette number. Put the cassette in a plastic container; add
50-100 mL fast decal (Fisher PROTOCOL
Decalfifier B). Let the specimen decal 20-30 minutes (set timer to remind).
Wash with tap water 5 minutes, then transfer to container with formalin. Submit
for tissue processor. Order “Panel” for
special stain.
Separate specimen of marrow
clot should be wrapped with Histowrap (blue paper) if the clot is not firm,
then submit entirely without decalcification.
Tissue is processed,
embedded, and cut. Each block has 2 slides stained with H & E, one with
Giemsa, and one with Iron. Order Iron
stain of one marrow aspirate, and satin 2 aspirate slides with Wright stain.
