学习了，谢谢周老师

  学习了，谢谢周老师。

 学习了，谢谢周老师。

 太厉害了，是从哪里来的呢？

 学习了

 好病例

 唉 真是够牛的

看来病理真是需要下大功夫啊

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 没有CK还真想不出转移癌

 没有周老师点评，真是没有想到，我想周老师出的病例，肯定是ML，真没想到，这对以后的工作是很有帮助的，没有不可能的，只有咱想不到的。考虑周全，多形组化分析。

 大间变

霍奇金

1)并不否定诊断转移性癌. 2)提供的病史似乎原发灶可能是胰腺,但"淋巴结癌细胞"并没有胰腺癌的形态学特点, 3)尽管形态学不象癌,IHC标记结果CK+诊断转移癌的正确率可能高达99.9%; 但是病理医师也应警惕0.1% 可能不是转移癌.我国是个人口大国,罕见病例的绝对 数应该高于国外,但是我国的报道却佷少,为什么? 4)DR.arhus很有经验,是否再深层次的讲解一下此例与"cytokeratin positive interstitial reticulum cell tumor"鉴别诊断.因为CPIRCT非常罕见,我国仅南京周晓军教授在中华医学 (英文版)上发表一例. 5)参考文献: 1. Kwon JE, Yang WI, Kim HK,et al. Cytokeratin-positive interstitial reticulum cell sarcoma: a case report with cytological, immunohistochemical, and ultrastructural findings. Cytopathology. 2009;20:202-5. 2.Dong YC, Wu B, Sheng Z, et al. Cytokeratin-positive interstitial reticulum cell tumors of lymph nodes: a case report and review of literature. Chin Med J (Engl). 2008;121:658-63. 3.Schuerfeld K, Lazzi S, De Santi MM, et al. Cytokeratin-positive interstitial cell neoplasm: a case report and classification issues. Histopathology. 2003;43:491-4. AIMS: Tumours of dendritic/accessory cell origin are rare neoplasms arising in lymph nodes. Among these, tumours derived from cytokeratin-positive interstitial reticulum cells (CIRCs), a subset of fibroblastic reticulum cells, are reported even less frequently. The International Lymphoma Study Group (ILSG) has recently proposed a classification for tumours of histiocytes and accessory dendritic cells in which CIRC tumours are not included. We report a case of a CIRC tumour arising in a submandibular lymph node of a 66-year-old male. METHODS AND RESULTS: The neoplasm was composed of spindle cells with elongated or round nuclei, prominent nucleoli and abundant cytoplasm. These cells were arranged in a diffuse fascicular and vaguely whorled pattern. The tumour cells stained diffusely for S100, vimentin, desmin, lysozyme, and focally for CD68 and cytokeratins 7, 8, 18, CK-AE1 and CK-pool. Electron microscopy was performed for further evaluation on samples taken from the paraffin block; this revealed cytoplasmic projections and rudimentary cell junctions. CONCLUSIONS: Histopathologist should be aware of the existence of tumours deriving from CIRCs, as these cases may be misdiagnosed as metastatic carcinoma. Careful clinical and pathological evaluation is necessary to exclude this possibility. 4.Lucioni M, Boveri E, Rosso R, et al. Lymph node reticulum cell neoplasm with progression into cytokeratin-positive interstitial reticulum cell (CIRC) sarcoma: a case study. Histopathology. 2003;43:583-91. AIMS: To detail on sequential biopsies the morphological and immunohistochemical features of a case of primary lymph nodal fibroblastic reticulum cell (FBRC) tumour which progressed into a clinically aggressive cytokeratin-positive interstitial reticulum cell (CIRC) sarcoma. METHODS AND RESULTS: A 70-year-old female underwent surgical excision of an enlarged submandibular lymph node. The nodal architecture was effaced by a neoplastic proliferation of medium to large cells, round to oval to spindle in shape, growing in a storiform pattern. The tumour stained for vimentin, CD68, factor XIIIa, alpha1-antitrypsin, fascin and actin. Dendritic and endothelial cell markers were negative. A diagnosis of FBRC tumour was made by combining pathological and clinical data. The patient received no therapy but 5 months later the tumour relapsed, exhibiting a deceptively pleomorphic cytology, phenotypic changes (strong cytokeratin positivity), intense p53 expression and aggressive clinical course with fatal outcome. In-situ hybridization for Epstein-Barr virus was negative. CONCLUSIONS: We speculate that the morphological changes and p53 expression of the relapsing neoplasm might reflect tumour cell dedifferentiation, in keeping with the aggressive clinical course. The intense p53 expression suggests that this oncoprotein might also play a role in reticulum cell tumorigenesis. 5. Chan AC, Serrano-Olmo J, Erlandson RA, Rosai J. Cytokeratin-positive malignant tumors with reticulum cell morphology: a subtype of fibroblastic reticulum cell neoplasm? Am J Surg Pathol. 2000;24:107-16. Cytokeratin-positive interstitial reticulum cells (CIRCs) have been described as a subset of fibroblastic reticulum cells (FBRCs) normally found in lymph nodes, the spleen, and tonsils. Although tumors derived form other reticulum (dendritic) cells, specifically follicular dendritic cells, interdigitating dendritic cells, and cytokeratin-negative FBRCs, have been well documented and are now accepted, this is not the case for tumors of CIRCs. A possible reason for this failure is the difficulty in distinguishing them from other tumors, particularly carcinoma. We report three cases of cytokeratin-positive malignant tumors with a reticulum cell morphology: two located in the mediastinum and one in the soft tissue in the proximal forearm. All cases coexpressed vimentin, and one case coexpressed smooth muscle actin and desmin, resulting in a phenotype similar to that of some normal CIRCs. Although metastatic carcinoma from an occult or regressed primary tumor cannot be excluded completely, we raise the possibility of a CIRC origin for these cases. 6. Gould VE, Bloom KJ, Franke WW, et al.Increased numbers of cytokeratin-positive interstitial reticulum cells (CIRC) in reactive, inflammatory and neoplastic lymphadenopathies: hyperplasia or induced expression? Virchows Arch. 1995;425:617-29. A total of 291 enlarged lymph nodes showing a range of reactive-inflammatory processes, primary and metastatic neoplasms were studied to determine the distribution and immunoprofile of their cytokeratin-positive interstitial reticulum cells (CIRC) in comparison with normal nodes. In 258/291 nodes (89%), CIRC numbers were distinctly increased in the subcapsular, paracortical and, occasionally, in the medullary zones; often, these increased CIRC formed networks around follicles, sinuses and vessels. CIRC had comparatively small, irregularly shaped bodies and dendritic processes; occasionally, giant forms were noted. CIRC contained cytokeratins (CK) 8 and 18 but not 19, as shown by immunohistochemistry, and by gel electrophoresis with subsequent immunoblotting. They co-expressed vimentin consistently, alpha-smooth-muscle actin frequently,and desmin less frequently. They did not contain desmoplakins, Factor VIII,S-100, LCA, B and T lymphocyte- and macrophage-associated antigens, chromogranin A, synaptophysin or the A-80 glycoprotein. We found no clear correlation between the increased CIRC and given nodal disease processes. However, CIRC were most abundant in nodes free of but draining malignant tumours; bizarre CIRC assemblies were noted in HIV lymphadenopathy. CIRC appear to represent a subset of the so-called "fibroblastic reticulum cells" of lymph nodes. Their function remains undetermined; their increase in diverse lymphadenopathies suggests that they partake in nodal reactions to injury. It remains unclear whether the increase in CIRC relative number is due to proliferation or to CK gene induction processes but their presence and potential capability to undergo hyperplasia with dysplastic forms should alert pathologists to possible diagnostic pitfalls. In addition, we discuss that CIRC may undergo transformation and represent the "cell of origin" of certain CK-positive tumours restricted to lymph nodes.

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 真没想到是转移癌！学习了，谢！

 学习了，谢谢！

 

以下是引用xljin8在2009-11-7 11:12:00的发言： 淋巴结内有CK+细胞并不一定都是转移癌, 例如纤维母细胞样网状细胞(Fibroblastic reticulum cell) 可表达CK和SMA.

是的。说得对！

1、从形态看，这些细胞像不像纤维母细胞样网状细胞？

2、从免疫表型看，纤维母细胞样网状细胞会不会表达如此强？会不会呈这样的分布形式？