浸润性小叶癌中见到两个小叶/导管增生性病变。每个病变不超过一个4倍视野。拍摄时使用的最低倍为10倍。

它们出现在同一张切片中。

图1 HE 10X

图2 HE 20X

图3 CK5/6 20X （背影太强，深棕色视为阳性，土黄色视为阴性）

图4 34BE12 20X

图5 E-Ca 20X

图6 ER 20X

图7 PR 20X

 另一个视野。

图1 HE 10X

图2 HE 20X

图3 HE 40X

图4 HE 40X

图5 CK5/6 20X （背影太强，深棕色视为阳性，土黄色视为阴性）

图6 34BE12 20X

图7 34BE12 20X

图8 E-Ca 20X

图9 ER 20X

图10 PR 20X

UDH

UDH generally displasys either diffuse or a mosaic pattern of positivity with high molecular weight cytokeratins[1963,2126] such as CK5, CK1/5/10/14 (clones CK34BetaE12 or clone D5/16 B4).

____from WHO 2003, Pg65

ADH----immunoprofile

Nearly 90% of ADH are negative for high molecular weight cytokeratins 1/5/10/14 (clones CK34BetaE12 and D5/16 B4), an important feature in separating ADH from UDH[1963,2126].

____from WHO 2003, Pg67

Hormone receptor expression

There is agreement that nearly all examples of ADH express high levels of ER in nearly all the cells[72,1301,2667]. The relationship between ER positive cell numbers and patient age, as found in normal breast epithelium, is lost in these ADH lesions, indicating autonomy of ER expression or of the cells expressing the receptor[2667].

____from WHO 2003, Pg71

Ref

73. Allred DC, Mohsin SK, Fuqua SA, et al. Histological and biological evolution of human premalignant breast disease. Endocr Relat Cancer, 2001, 8:47-61

1301. Iqbal M, Davies MP, Shoker BS, et al. Subgroups of non-atypical hyperpasia of breast difined by proliferation of oestrogen receptor-positive cells. J Pathol, 2001, 193:333-338 

1963. Nagle RB, Bocker W, Davis JR, et al. Characterization of breast carcinomas by two monoclonal antibodies distingushing myoepithelial from luminal epithelial cells. J Histochem Cytochem, 1986, 34: 869-881

2126. Otterbach F, Bankfalvi A, Berger S, et al. Cykeratin 5/6 immunohistochemistry assists th edifferential diagonsis of atypical proliferation of the breast. Histopathology, 2000,37:232-240

2667. Shoker BS, Jarvis C, Sibson DR, et al. Oestrogen receptor expression in the normal and precancerous breast. J Pathol, 1999, 188:237-244

PS：

We are not access to the original papers in China for further study. What a pity!

Thanks!

abin