This Pap shows many hyperchromatic crowed groups (HCG) with increase N/C ratio, dark nuclei, but with evenly distributed chromatin and wiout nuclear membrane irregularity. There is one mitotic figure or apopotosis in the fig 2. The differential diagnoses are HSIL, immature squamous metaplasia and horman related change (some ones mention above). If it was a liquid based cytoloy, you can order high risk HPV testing and also make a cell block using the residual specimen. The chance of high grade lesion is very low if HR HPV is negative. If you are luck to have a good cell block you can order ki67 and p16. The twe immuno stains are most useful for differential diagnosis of c2+ in cervical biopsy specimen. C2+ squamous epithelia show moderately or markedly increased proliferative index and  diffuse stain for P16. Some times cell bloks can also contain large fragments of cervical mucosa. I had good experience for some cases in the past half year.

This is a conventional Pap smear and you will not have additonal material for these tests. It is better to diagnose ASC-H and to suggest a colposcopy.

Your photos are nice.

In the US 90-95% of the Paps are LBC (ThinPrep or Sure Path or few others), 98% LBC paps in our lab.

Just wonder what percentage of Pap tests are LBC in China.

Thanks,

这么多深染的核，看似很恶确无核内结构。

深染的片子看起来很麻烦。

ASC-US