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我的问题能得到这么多老师的回答,感激万分,多谢各位好老师的指导。以下是说明书上的内容摘录。
rabbit anti-connexin 43
Qty:50ug/2OOul
catalog NO.71-0700
lot NO.50393484
FORM: this polyclonal antibody is supplied as a 200ul aliquot of affinity purufied anibody at a concentrarion of 0.25mg/ml in 10mM ohosphate buffered saline,PH7.4,congtaining 0.1%NaN3.
USAGE:IHC(paraffin-embedded):1-5ug/ml.
我欲将其配制稀释度为1:100的浓度,我的配制方法如下,请楼上各位老师指教:
1:100:1ul试剂+99ul 0.1%PBS?
另问可用抗体稀释液稀释吗?用抗体稀释液最多能保存多长时间?