正常乳腺组织，炎症，良性肿瘤时这些免疫组化指标会发生什么样的改变呢？

 Cytokeratin(HMW)-34BE12 应该在正常组织和导管癌中均可以表达，曾经用组织芯片进行标记过。
E－cadherin比较有用，我们常规用其鉴别导管癌和小叶癌，但确实遇到1例小叶癌阳性的。
β－catenin没有用过，以后试试。
谢谢LZ的奉献！

 一直再用这些抗体做二者的鉴别。至于少数E-cad阳性的小叶癌，不知是否有导管癌伴小叶癌化（cancerization）的问题。

Histology of some ductal carcinomas mimics lobular carcinomas, and this is observed at both in situ and invasive cases. The most useful marker to differentiate ductal from lobular differentiation remains E=Cadherin. Very rare cases of lobular carcinoma (in situ and invasive) will express strong E-Cadherin on immunohistochemistry. As for 34betaE12, it is not as consistent as some have claimed. In other words, some ductal carcinomas have strong expression of this marker just like lobular carcinomas do. My experience with beta-catenin is limited.

翻译并学习马老师的发言：

 在导管原位癌和浸润癌的病例中均可看到某些导管癌模仿小叶癌的组织学特点。区别导管和小叶分化的最有用的抗体是E－Cadherin。极少有小叶管癌（原位的和浸润的）会有E-Cadherin 的强表达。至于34betaE12，它和某些人声称那样并不一致。换言之，某些导管癌和小叶癌一样，有这个抗体的强表达。我对于beta-catenin 的经验有限。

 谢谢knight，我是随便翻译的，对不起，应该abin有个分工的，免得劳动重复，呵呵。

 不要这么客气。我不知道你已经翻译过了。大家一起学习，我也是在练习。Abin指定的人没来，我先抛砖引玉他应该不会生气的。呵呵。

 

可能小白， 猪猪和 女巫最近忙没有上网看到翻译任务。毕竟大家都有忙的时候，都可能需要别的朋友援助。非常感谢wangzhen_01和knight及时帮助他们完成。朋友们能互相合作，分工不分家，我非常开心。再次感谢！

楼上两位发表的时间只有几分钟之差，呵呵，可能发表时都看不到对方已经发表了。而且，我分工不明，检讨。

 最近美国外科病理学杂志[American Journal of Surgical Pathology. 31(3):427-437, March 2007)],:Lobular Versus Ductal Breast Neoplasms: The Diagnostic Utility of P120 Catenin.
但是只能看摘要，无法看全文。

 

文章还可以，我放在huaxiapathology@yahoo.com.cn。供大家参考。

收到wangzhen_01 老师发来的文献，在此特表感谢。

  感谢yang老师提供信息，感谢wangzhen_01查阅原文。

Lobular Versus Ductal Breast Neoplasms

The Diagnostic Utility of P120 Catenin

Abstract: The distinction between lobular and ductal lesions of the breast is important in several circumstances.
Diagnostic reproducibility of lobular versus ductal lesions, based on histology alone, is less than optimal. The
proper distinction between atypical lobular hyperplasia, lobular carcinoma in situ and low-grade ductal carcinoma
in situ is critical for patient management. Patients who have a core biopsy of invasive lobular carcinoma often
have preoperative magnetic resonance imaging to prepare the surgeon for proper margin attainment. E-cadherin, a
negative membrane marker for lobular neoplasia, is useful in the distinction of lobular versus ductal neoplasia,
but as a negative marker, can be difficult to interpret in particularly challenging cases. In this study, we
surveyed primary and metastatic ductal lesions (62) and lobular lesions (64) of the breast to determine if P120
catenin is useful in the diagnostic distinction between lobular and ductal neoplasia. Primary breast ductal and
lobular preneoplastic and neoplastic lesions were immunostained with E-cadherin and P120ctn and independently
classified as ductal or lobular lesions. In addition, a wide array of carcinomas of different types were surveyed
with P120ctn in tissue microarrays to ascertain whether the cytoplasmic P120ctn immunostaining pattern observed in
lobular neoplasia was unique. Accurate categorization of ductal versus lobular neoplasia in the breast with
P120ctn immunostaining was effective in all cases. Separation of low-grade ductal carcinoma in situ from lobular
neoplasia was efficient. Diagnostically, P120ctn was particularly useful in identifying early lesions of lobular
neoplasia. Of the other tumors that may morphologically mimic lobular carcinoma, only the diffusely infiltrating
variants of rectal and gastric carcinomas showed diffuse cytoplasmic P120ctn immunostaining. Caution should be
exercised when examining tumors in metastatic sites with P120ctn, with the incorporation of an appropriate panel
of immunostains. 

 

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http://www.ipathology.org.cn/forum/forum_display.asp?keyno=2029
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http://www.ipathology.org.cn/forum/forum_display.asp?keyno=29908

http://www.ipathology.org.cn/forum/forum_display.asp?keyno=62115

 

 学习了，谢谢

 我近来用组织芯片回顾了153例乳腺癌，发现P120Catenin和E-Catenin在乳腺小叶癌和导管癌的鉴别上特异性很好，CK（high）-34βE12特异性不高。过一段时间我把照片和结果上传。

 学习了