请教和求助:恶性黑色素瘤会出现CD99阳性吗?

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楼主发表于 2008-03-30 23:40|举报|关注(0)
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各位老师好！请教和求助:恶性黑色素瘤会出现CD99阳性吗?机理是什么？神经分化？

并请提供参考资料，多谢！！！

我有1例食管恶性黑色素瘤CD99阳性，现在找不到文献，敬请老师们帮忙！

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本帖最后由于 2008-03-31 12:49:00 编辑

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: 广州金域病理

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1 楼发表于2008-03-31 22:30:00举报|引用
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可以的，CD99太泛滥了，特异性差。

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华夏病理/粉蓝医疗

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2 楼发表于2008-03-31 23:22:00举报|引用
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以下是引用abin在2008-3-31 22:30:00的发言：

可以的，CD99太泛滥了，特异性差。

请abin帮我找找文献:恶性黑色素瘤CD99的表达.多谢!

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: 广州金域病理

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3 楼发表于2008-04-02 21:40:00举报|引用
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遵命：有3篇英文，未查到中文。

1: J Cutan Pathol. 2007 Jul;34(7):576-80. Links
Differentiating spitzoid melanomas from Spitz nevi through CD99 expression.
CD99表达鉴别Spitz样黑色素瘤和Spitz痣
King MS, Porchia SJ, Hiatt KM.
Department of Pathology, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA.

BACKGROUND: A true diagnostic marker differentiating Spitz nevi (SN) from spitzoid melanoma (sMM) has been elusive. CD99, a transmembrane glycoprotein, believed to play a role in many neoplastic processes, has yet to be investigated in this regard. Recently, the expression of CD99 has been shown in 60% of primary melanomas. Other studies exploring the expression of CD99 in melanocytic lesions have not been performed. Here, we evaluate the presence of CD99 in these two histologically difficult to differentiate entities. METHODS: Cases of sMM were selected based on the presence of key microscopic words: spitz, spindled and/or epitheliod. In addition, all the cases of SN over the past 7 years were retrieved. Each case was stained with anti-human CD99 and analyzed for the presence of CD99 staining. RESULTS: Fifteen of 27 cases (56%) of sMM expressed CD99 compared with only 3 of 58 cases (5%) of SN. CONCLUSIONS: This study shows 56% of sMM and only 5% of SN express CD99. Eight of the sMM showed strong diffuse staining, a pattern not seen in any of the SN. This study does not elucidate the role that CD99 plays in these melanocytic processes; however, it does show that CD99 can be a useful tool in distinguishing sMM from SN.
背景：很难把握真正有助于区分Spitz样黑色素瘤(sMM)和Spitz痣(SN)的免疫组化标记物。CD99是一种跨膜糖蛋白，被认为在肿瘤过程中起作用，我们用CD99对这个问题进行了研究。最近发现CD99在原发恶黑中表达率为60%。还没有其它关于CD99在黑色素细胞病变中的表达。在此，我们评价了CD99在这两种组织学上难以鉴别的病变中的表达。方法：根据以下显微镜下描述的关键字筛选sMM病例：spitz，梭形和/或上皮样。另外，检索最近7年内所有SN病例。每个病例都用抗人-CD99染色，并分析CD99染色结果。结果：15/27例(56%)sMM表达CD99，而SN仅有3/58（5%）。结论：本研究显示56%的sMM表达CD99，而SN中的表达仅5%。8例sMM呈弥漫强阳性，这种表达不见于SN。本研究不能解释CD99在黑色素细胞病变过程中的作用，然而，它确实提示CD99可能是区分sMM和SN的有用工具。
PMID: 17576338 [PubMed - indexed for MEDLINE]

Am J Dermatopathol. 2007 Apr;29(2):169-71. Links
A metastatic melanoma with an unusual immunophenotypic profile.
一例转移性黑色素瘤伴少见免疫表型
Gao Z, Stanek A, Chen S.
Department of Pathology, Albert Einstein College of Medicine North Shore Long Island Jewish Health System, New Hyde Park, NY 11040, USA.

The most commonly used melanocytic markers are S100, HMB45, Melan-A, or MART-1 and tyrosinase. Melanoma with complete, concordant loss of these markers has not been reported. We report a case of metastatic melanoma with complete loss of staining for S100, HMB45, Melan-A, and tyrosinase. Interestingly, both the primary melanoma and its metastasis were strongly positive for CD99.
最常用的黑色素细胞标记物是S100、HMB45、Melan-A或MART-1，和酪氨酸酶。完全、一致丢失以上表达的黑色素瘤还未见报道。我们报道一例转移性黑色素瘤完全丢失S100、HMB45、Melan-A和酪氨酸酶。有趣的是，这例原发灶和转移灶都呈CD99强阳性。
PMID: 17414440 [PubMed - indexed for MEDLINE]

J Cutan Pathol. 2006 Oct;33(10):663-6. Links
Immunoreactivity of CD99 in invasive malignant melanoma.
CD99在浸润性恶黑中的免疫反应
Wilkerson AE, Glasgow MA, Hiatt KM.
Department of Pathology, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA.

BACKGROUND: CD99, also known as p30/32, is a glycoprotein product of the MIC2 gene. It was originally utilized in immunohistochemistry as a unique marker for Ewing sarcoma, other primitive neuroectodermal tumors, and subsequently in other tumors. Its expression in malignant melanoma (MM) has not been well documented, with just two isolated cases of MM recently reported. Recent studies have documented CD99 expression in a significant percentage of atypical fibroxanthomas (AFX), posing potential diagnostic problems in differentiating these two entities. As mistaking MM for AFX based on immunohistochemical staining pattern has significant consequences, we sought to determine the percentage of invasive MM in our archives that have this staining pattern. METHODS: Seventy-eight cases of invasive melanoma were retrieved from our files. Each case was stained with mouse anti-human CD99 and evaluated for membranous expression. RESULTS: Our evaluation revealed that 47 of 78 MM cases (60%) stain positive for CD99. CONCLUSION: This study is the first to demonstrate, in a large series, the prevalence of CD99 expression in primary cutaneous melanoma. Additionally, this introduces in the histologic differential diagnosis of CD99 expressing dermal spindle cell lesions.
背景：CD99也称为p30/32，是一种糖蛋白，是MIC2基因的表达产物。它最初用作Eing肉瘤、PNET的独特标记，以后又用于其它肿瘤。有关它在恶黑（MM）中表达的文献不多，最近仅报道两例散发MM。最近研究发现，CD99在很多病变中有显著的表达率，如不典型纤维黄色瘤（AFX），从而提出潜在的诊断问题：如何区分这两者。因为把根据免疫组化把MM误诊为AFX会产生严重后果，我们决定检测浸润性恶黑中CD99的表达率。方法：从我们的文献中检索出78例浸润性恶黑。每例均作鼠抗-人CD99并评价膜表达情况。结果：47/78例（60%）CD99阳性。结论：本研究第一次用大宗病例证实，CD99在原发性皮肤黑色素瘤的表达率。另外，还介绍了CD99阳性的皮肤梭形细胞病变的组织学鉴别诊断。
PMID: 17026517 [PubMed - indexed for MEDLINE]

遵命：有3篇英文，未查到中文。

1: J Cutan Pathol. 2007 Jul;34(7):576-80. Links
Differentiating spitzoid melanomas from Spitz nevi through CD99 expression.
CD99表达鉴别Spitz样黑色素瘤和Spitz痣
King MS, Porchia SJ, Hiatt KM.
Department of Pathology, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA.

BACKGROUND: A true diagnostic marker differentiating Spitz nevi (SN) from spitzoid melanoma (sMM) has been elusive. CD99, a transmembrane glycoprotein, believed to play a role in many neoplastic processes, has yet to be investigated in this regard. Recently, the expression of CD99 has been shown in 60% of primary melanomas. Other studies exploring the expression of CD99 in melanocytic lesions have not been performed. Here, we evaluate the presence of CD99 in these two histologically difficult to differentiate entities. METHODS: Cases of sMM were selected based on the presence of key microscopic words: spitz, spindled and/or epitheliod. In addition, all the cases of SN over the past 7 years were retrieved. Each case was stained with anti-human CD99 and analyzed for the presence of CD99 staining. RESULTS: Fifteen of 27 cases (56%) of sMM expressed CD99 compared with only 3 of 58 cases (5%) of SN. CONCLUSIONS: This study shows 56% of sMM and only 5% of SN express CD99. Eight of the sMM showed strong diffuse staining, a pattern not seen in any of the SN. This study does not elucidate the role that CD99 plays in these melanocytic processes; however, it does show that CD99 can be a useful tool in distinguishing sMM from SN.
背景：很难把握真正有助于区分Spitz样黑色素瘤(sMM)和Spitz痣(SN)的免疫组化标记物。CD99是一种跨膜糖蛋白，被认为在肿瘤过程中起作用，我们用CD99对这个问题进行了研究。最近发现CD99在原发恶黑中表达率为60%。还没有其它关于CD99在黑色素细胞病变中的表达。在此，我们评价了CD99在这两种组织学上难以鉴别的病变中的表达。方法：根据以下显微镜下描述的关键字筛选sMM病例：spitz，梭形和/或上皮样。另外，检索最近7年内所有SN病例。每个病例都用抗人-CD99染色，并分析CD99染色结果。结果：15/27例(56%)sMM表达CD99，而SN仅有3/58（5%）。结论：本研究显示56%的sMM表达CD99，而SN中的表达仅5%。8例sMM呈弥漫强阳性，这种表达不见于SN。本研究不能解释CD99在黑色素细胞病变过程中的作用，然而，它确实提示CD99可能是区分sMM和SN的有用工具。
PMID: 17576338 [PubMed - indexed for MEDLINE]

Am J Dermatopathol. 2007 Apr;29(2):169-71. Links
A metastatic melanoma with an unusual immunophenotypic profile.
一例转移性黑色素瘤伴少见免疫表型
Gao Z, Stanek A, Chen S.
Department of Pathology, Albert Einstein College of Medicine North Shore Long Island Jewish Health System, New Hyde Park, NY 11040, USA.

The most commonly used melanocytic markers are S100, HMB45, Melan-A, or MART-1 and tyrosinase. Melanoma with complete, concordant loss of these markers has not been reported. We report a case of metastatic melanoma with complete loss of staining for S100, HMB45, Melan-A, and tyrosinase. Interestingly, both the primary melanoma and its metastasis were strongly positive for CD99.
最常用的黑色素细胞标记物是S100、HMB45、Melan-A或MART-1，和酪氨酸酶。完全、一致丢失以上表达的黑色素瘤还未见报道。我们报道一例转移性黑色素瘤完全丢失S100、HMB45、Melan-A和酪氨酸酶。有趣的是，这例原发灶和转移灶都呈CD99强阳性。
PMID: 17414440 [PubMed - indexed for MEDLINE]

J Cutan Pathol. 2006 Oct;33(10):663-6. Links
Immunoreactivity of CD99 in invasive malignant melanoma.
CD99在浸润性恶黑中的免疫反应
Wilkerson AE, Glasgow MA, Hiatt KM.
Department of Pathology, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA.

BACKGROUND: CD99, also known as p30/32, is a glycoprotein product of the MIC2 gene. It was originally utilized in immunohistochemistry as a unique marker for Ewing sarcoma, other primitive neuroectodermal tumors, and subsequently in other tumors. Its expression in malignant melanoma (MM) has not been well documented, with just two isolated cases of MM recently reported. Recent studies have documented CD99 expression in a significant percentage of atypical fibroxanthomas (AFX), posing potential diagnostic problems in differentiating these two entities. As mistaking MM for AFX based on immunohistochemical staining pattern has significant consequences, we sought to determine the percentage of invasive MM in our archives that have this staining pattern. METHODS: Seventy-eight cases of invasive melanoma were retrieved from our files. Each case was stained with mouse anti-human CD99 and evaluated for membranous expression. RESULTS: Our evaluation revealed that 47 of 78 MM cases (60%) stain positive for CD99. CONCLUSION: This study is the first to demonstrate, in a large series, the prevalence of CD99 expression in primary cutaneous melanoma. Additionally, this introduces in the histologic differential diagnosis of CD99 expressing dermal spindle cell lesions.
背景：CD99也称为p30/32，是一种糖蛋白，是MIC2基因的表达产物。它最初用作Eing肉瘤、PNET的独特标记，以后又用于其它肿瘤。有关它在恶黑（MM）中表达的文献不多，最近仅报道两例散发MM。最近研究发现，CD99在很多病变中有显著的表达率，如不典型纤维黄色瘤（AFX），从而提出潜在的诊断问题：如何区分这两者。因为把根据免疫组化把MM误诊为AFX会产生严重后果，我们决定检测浸润性恶黑中CD99的表达率。方法：从我们的文献中检索出78例浸润性恶黑。每例均作鼠抗-人CD99并评价膜表达情况。结果：47/78例（60%）CD99阳性。结论：本研究第一次用大宗病例证实，CD99在原发性皮肤黑色素瘤的表达率。另外，还介绍了CD99阳性的皮肤梭形细胞病变的组织学鉴别诊断。
PMID: 17026517 [PubMed - indexed for MEDLINE]