Sox10-a marker for not only schwannian and melanocytic neoplasms but also myoepithelial cell tumors of soft tissue: a systematic analysis of 5134 tumors.

Sox10，一种不仅标记施旺细胞及黑素细胞肿瘤、而且可以标记软组织肌上皮肿瘤的标记物：5134例肿瘤的全面分析

Miettinen M,McCue PA,Sarlomo-Rikala M,Biernat W,Czapiewski P,Kopczynski J,Thompson LD,Lasota J,Wang Z,Fetsch JF
American journal of surgical pathology； Volume 39, June 2015,Issue 6,pp: 729-872:826-35  


Abstract

Sox10 transcription factor is expressed in schwannian and melanocytic lineages and is important in their development and can be used as a marker for corresponding tumors. In addition, it has been reported in subsets of myoepithelial/basal cell epithelial neoplasms, but its expression remains incompletely characterized. In this study, we examined Sox10 expression in 5134 human neoplasms spanning a wide spectrum of neuroectodermal, mesenchymal, lymphoid, and epithelial tumors. A new rabbit monoclonal antibody (clone EP268) and Leica Bond Max automation were used on multitumor block libraries containing 30 to 70 cases per slide. Sox10 was consistently expressed in benign Schwann cell tumors of soft tissue and the gastrointestinal tract and in metastatic melanoma and was variably present in malignant peripheral nerve sheath tumors. In contrast, Sox10 was absent in many potential mimics of nerve sheath tumors such as cellular neurothekeoma, meningioma, gastrointestinal stromal tumors, perivascular epithelioid cell tumor and a variety of fibroblastic-myofibroblastic tumors. Sox10 was virtually absent in mesenchymal tumors but occasionally seen in alveolar rhabdomyosarcoma. In epithelial tumors of soft tissue, Sox10 was expressed only in myoepitheliomas, although often absent in malignant variants. Carcinomas, other than basal cell-type breast cancers, were only rarely positive but included 6% of squamous carcinomas of head and neck and 7% of pulmonary small cell carcinomas. Furthermore, Sox10 was often focally expressed in embryonal carcinoma reflecting a primitive Sox10-positive phenotype or neuroectodermal differentiation. Expression of Sox10 in entrapped non-neoplastic Schwann cells or melanocytes in various neoplasms has to be considered in diagnosing Sox10-positive tumors. The Sox10 antibody belongs in a modern immunohistochemical panel for the diagnosis of soft tissue and epithelial tumors.


摘要

在施旺细胞和黑素细胞系中表达的Sox10转录因子在其发育中非常重要，并可作为其相应肿瘤的标记物。另外，已有Sox10在肌上皮/基底细胞肿瘤亚群中的报道，但其表达仍然没有被完全阐明。本研究在神经外胚层肿瘤、间叶源性肿瘤、淋巴系统肿瘤及上皮性肿瘤在内的5134个人类肿瘤中检测了Sox10的表达。每片包含30-70例的多肿瘤组织芯片运用一种新的兔单克隆抗体(克隆号：EP268)和莱卡自动化仪进行检测。Sox10在发生于软组织和胃肠道的良性施旺细胞瘤及转移性黑色素瘤中具有一致性表达，而在恶性外周神经鞘膜瘤中具有不同程度的表达。相反，Sox10在许多潜在的类似神经鞘瘤的肿瘤中却不表达，例如细胞性神经鞘粘液瘤、脑膜瘤、胃肠道间质瘤、血管周上皮样细胞肿瘤和各种各样的纤维母/肌纤维母细胞肿瘤。事实上Sox10在间叶源性肿瘤不表达，偶尔在腺泡状横纹肌肉瘤中表达。在软组织上皮性肿瘤中，Sox10仅在肌上皮瘤中表达，而在恶性变异型肿瘤中不表达。此外，Sox10在胚胎性癌中常局灶性表达，表明了其具有原始Sox10-阳性表型或具有向神经外胚层分化的潜能。在诊断Sox10阳性肿瘤时，必须考虑到夹杂在多种肿瘤内的非肿瘤性施旺细胞或黑色素细胞表达Sox10。因此在软组织和上皮性肿瘤的诊断中，Sox10应该被加入进免疫组织化学抗体组中。