Pathol Int. 2015 Mar;65(3):119-25. doi: 10.1111/pin.12252. Epub 2015 Jan 19.

HBME-1 and CD15 immunocytochemistry in the follicular variant of thyroid papillary carcinoma.

Ohta M1, Ookoshi T, Naiki H, Imamura Y.
Abstract

Papillary carcinoma is the most common thyroid malignancy. As the cytological diagnosis of papillary carcinoma is not difficult in patients with the usual type of lesion, fine-needle aspiration (FNA) cytology is an effective method for preoperative evaluation. However, this modality is often ineffective in identifying the follicular variant of papillary thyroid carcinoma (FVPTC) due to its similarity to other follicular lesions and the incompleteness of typical nuclear features. Therefore, we investigated the expression of immunocytochemical markers of papillary carcinoma in cytological specimens of FVPTC and evaluated their utilities. The immunoreactivity of HBME-1 and CD15 was investigated using 50 imprint smear cytological specimens obtained from thyroid lesions, including 13 FVPTC. The sensitivity and specificity of HBME-1 for FVPTC were 92% and 89%, respectively, while those of CD15 were 23% and 100%, respectively. In conclusion, HBME-1 is a sensitive marker of papillary carcinoma, including both usual type and FVPTC, in cytological specimens. Therefore, using HBME-1 immunocytochemistry in FNA cytology will lead to reduction of the incidence of false-negative diagnoses of FVPTC. Although CD15 is apparently inferior in terms of sensitivity for FVPTC, its excellent specificity will support the definitive diagnosis of thyroid malignancies, including FVPTC, after screening with HBME-1.
摘要   乳头状癌是甲状腺最常见的恶性肿瘤，通过细胞学诊断普通类型的乳头状癌并不困难，细针抽吸活检（FNA）细胞学是术前评估甲状腺病变一种非常有效的方法。然而由于滤泡变异亚型甲状腺乳头状癌（FVPTC）与其它滤泡性病变在细胞学上相似，且不完全具有典型乳头状癌核的特征，因此这种方法通常不能识别FVPTC。作者在FVPTC的细胞学标本中研究了乳头状癌免疫细胞化学标记物的表达，并评估了其应用价值。通过对可获得的甲状腺病变细胞标本，其中包括13例FVPTC，研究了HBME-1和CD15的免疫反应性。HBME-1对FVPTC的敏感性和特异性分别为92%、89%，而CD15对FVPTC的敏感性和特异性分别为23%、100%。总之，对细胞学标本，HBME-1 是诊断普通型乳头状癌和FVPTC一种非常敏感的标记物，因此，在FNA细胞学中使用HBME-1免疫细胞化学将减少FVPTC诊断的假阴性率。尽管CD15在诊断FVPTC敏感性方面较差，然而通过HBME-1筛选后，利用其较高的特异性能够明确甲状腺恶性肿瘤（包括FVPTC）的诊断。