Chromophobe hepatocellular carcinoma with abrupt anaplasia: a proposal for a new subtype
of hepatocellular carcinoma with unique morphological and molecular features

Mod Pathol 2013 (In press)

Hepatocellular carcinomas exhibit heterogeneous morphologies by routine light microscopy. Although some morphologies represent insignificant variations in growth patterns, others may represent unrecognized
subtypes of hepatocellular carcinoma. Identification of these subtypes could lead to separation of
hepatocellular carcinomas into discrete groups with unique underlying genetic changes, prognosis, or
therapeutic responses. In order to identify potential subtypes, two pathologists independently screened a
cohort of 219 unselected hepatocellular carcinoma resection specimens and divided cases into potential
subtypes. One of these promising candidate subtypes was further evaluated using histological and molecular
techniques. This subtype was characterized by a unique and consistent set of histological features: smooth
chromophobic cytoplasm, abrupt focal nuclear anaplasia (small clusters of tumor cells with marked nuclear
anaplasia in a background of tumor cells with bland nuclear cytology), and scattered microscopic
pseudocysts—we designate this variant as ‘chromophobe hepatocellular carcinoma with abrupt anaplasia’.
Thirteen cases were identified (6% of all hepatocellular carcinomas), including 6 men and 7 women with an
average age of 61 years. Six cases occurred in cirrhotic livers. Serum AFP was elevated in 6 out of 10 cases.
There were a variety of underlying liver diseases, but cases were enrichment for chronic hepatitis B, P¼0.006.
Interestingly, at the molecular level, this variant was strongly associated with the alternative lengthening
of telomere (ALT) phenotype by telomere FISH. ALT is a telomerase-independent mechanism of telomere
maintenance and is found in approximately 8% of unselected hepatocellular carcinomas. In contrast, 11/12
(92%) of the cases of chromophobe hepatocellular carcinoma with abrupt anaplasia were ALT-positive. In
summary, we propose that chromophobe hepatocellular carcinoma with abrupt anaplasia represents a new
subtype of hepatocellular carcinoma with unique morphological and molecular features.
Modern Pathology advance online publication, 3 May 2013; doi:10.1038/modpathol.2013.68