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- POX染色阳性，流式结果MPO阴性，是髓系还是淋系呢？
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这是一例髓系白血病。你的POX 染色是最可靠的证据。这不可能是假阳性。这个年龄的病人T-ALL 是很少见的。 流式中CD13, CD33 and CD117 都是可靠的证据。我们没有看到流式的原图，也不能过多的评价。许多髓系白血病都可表大CD7. 胞浆染色， 象MPO, TdT, cCD3, cKappa, cLambda, cCD22 有时在技术上和分析上有一定的困难。 下面是一段关于CD117 的文章。
Usefulness of Anti-CD117 in the Flow Cytometric Analysis
of Acute Leukemia （Am J Clin Pathol 2002;117:301-305）
Christine P. Hans, MD, William G. Finn, MD, Timothy P. Singleton, MD,*
Bertram Schnitzer, MD, and Charles W. Ross, MD
We assessed the diagnostic usefulness of adding
anti-CD117 to our existing flow cytometric profile in
the analysis of 150 consecutive cases of acute leukemia
(de novo or relapsed acute myelogenous leukemia
[AML], AML arising in myelodysplastic syndrome,
blast crisis of chronic myelogenous leukemia [CML],
acute lymphoblastic leukemia, acute unclassifiable
leukemia, and biphenotypic leukemia). CD117 was
expressed on more than 10% of blasts in 64% of de
novo AMLs (42/66), 95% of relapsed AMLs (19/20),
75% of AMLs arising from a myelodysplastic syndrome
(6/8), and 25% of myeloid blast crisis in CMLs (1/4).
CD117 was not expressed in acute lymphoblastic, acute
biphenotypic, or unclassified leukemia or lymphoid
blast crisis of CML. The specificity, positive predictive
value, sensitivity, and negative predictive value of
CD117 for AML were 100%, 100%, 69%, and 62%,
respectively. CD117 is a specific marker for
myeloblastic leukemias. Sensitivity is greatest in
French-American-British M2 and relapsed AML.
Intensity of CD117 expression is dim. Despite the high
specificity and positive predictive value, the addition of
anti-CD117 to our panel did not prove essential for the
assignment of blast lineage.
