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- 请教专家：Cyclin D1可见于哪些淋巴瘤？感激！
详细病例图片及免疫组化敬请链接：
http://www.ipathology.cn/channel/detail/name/bbs/article/405192.html
Cycin D1(+)、Ki-67 80%(+)、CD5(-)、Bcl-2(-)、Bcl-6(-)、CD20(+)、CD79a（-）、MUM-1（-）、CD10(-)、CD23（-）、CD3(-)、CD4RO(-)、CD45(+)、CK(-)。
免疫组化结果让人很是纠结，还请专家赐教！感谢！
标签：
- 重归学生时代！
×参考诊断
1. Semin Diagn Pathol. 2011 Aug;28(3):245-55.
Mantle cell lymphoma: recent insights into pathogenesis,
clinical variability,and new diagnostic markers.
Sander B.Division of Pathology, Department of Laboratory Medicine,
Karolinska Institutet,
Stockholm, Sweden. birgitta.sander@ki.se Mantle cell lymphoma (MCL; previously called centrocytic
lymphoma or lymphocytic lymphoma of intermediate differentiation) is a distinct
subtype of B-cell lymphoma, accounting for approximately 3%-10% of all lymphoma
diagnoses. The name refers to the growth pattern in early disease presentation
resembling the normal mantle zone that surrounds the germinal center of the
B-cell follicle. The hallmark of MCL is the t(11;14)(q13;q32), resulting in
aberrant expression of the CCND1 gene and expression of cyclin D1 in the tumor
cells. Expression and genomic profiling of MCL have provided new insight into
the pathogenesis and will be summarized in this review. Pitfalls in the differential diagnosis versus
B-cell chronic lymphocytic leukemia, B-cell prolymphocytic leukemia, cyclin
D1-positive diffuse large B-cell lymphoma, hairy cell leukemia, and plasma cell
tumors will be discussed, including the usefulness new diagnostic
markers SOX11 and CD200. In situ MCL, MCL with an indolent clinical course, and
cyclin D1-negative MCL are other topics of this review.
2. Leuk Lymphoma. 2011 Mar;52(3):458-66. Epub 2011 Feb 1.
Cyclin D1 positive diffuse large B-cell lymphoma is a post-germinal center-type lymphoma without alterations in the CCND1 gene locus.Vela-Chávez T, Adam P, Kremer M, Bink K, Bacon CM, Menon G, Ferry JA, Fend F, Jaffe ES, Quintanilla-Martínez L.
Institute of Pathology, University of Tübingen,
Comprehensive Cancer Center,Tübingen, Germany.
Comment in Leuk Lymphoma. 2011 Mar;52(3):358-9.
The aims of this study were to analyze the incidence and
morphology of cyclin D1+ DLBCL and cases of Richter transformation (RT), and to
elucidate possible molecular mechanisms of cyclin D1 overexpression.
Seventy-two cases of de novo DLBCL and 12 cases of RT were included in this
study. Cyclin D1 positivity was found in 10/66 (15%) cases of unselected de
novo DLBCL and in 2/11 (18%) cases of RT. Seven independently identified cases
of cyclin D1+ DLBCL, including one RT, were added to the study. Centroblastic
morphology was found in 17/19 (89%) cases of cyclin D1+, most with a
post-germinal center phenotype (CD10-, BCL6+, MUM1+). No alterations in the
CCND1 gene indicative for a translocation t(11;14) were identified by FISH.
Analysis of the MYC locus yielded gene copy alterations in five cases and no
disruption of the gene locus in any case, suggesting an alternative mechanism
of cyclin D1 deregulation.
3. J Clin Pathol. 2009 Sep;62(9):855-7.
Cyclin
D1 positive follicular lymphoma. Olsen B, Srkalovic G, Law M, McPhail E.
Ingham Regional Medical Center, Lansing, Michigan 48910,
USA.brian.olsen@irmc.org
Erratum in J Clin Pathol.
2009 Nov;62(11):1056. Hall, M [corrected to Law, M].This report describes an unusual case of cyclin D1
expression by an otherwise typical follicular lymphoma, of low histological grade.
BCL2-IGH and CCND1-IGH fusions were identified by interphase fluorescence in
situ hybridisation.
4. Appl Immunohistochem Mol Morphol. 2009 May;17(3):255-8. CD5 negative, Cyclin D1-positive diffuse large B-cell lymphoma (DLBCL) presenting as ruptured spleen. Teruya-Feldstein J, Gopalan A, Moskowitz CH. Department of Pathology, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA. feldstej@mskcc.org
We present a case of diffuse large B-cell lymphoma CD5
negative, Cyclin D1 positive presenting as ruptured spleen in a 63-year-old man
requiring emergent splenectomy. Tumor cells showed marked pleomorphism,
anaplasia, and increased mitotic figures with positive Cyclin D1, BCL6, MUM1,
P53, and a high MIB1 proliferative fraction. The patient received multiple
therapies and ultimately died. This case raises the differential diagnoses of
pleomorphic mantle cell lymphoma and other aggressive lymphomas with
pleomorphic, anaplastic, and Reed-Sternberg-like cells.
5. Histopathology. 2008 Jun;52(7):900-3. Cyclin
D1-positive diffuse large B-cell lymphoma.
Rodriguez-Justo M, Huang Y, Ye H, Liu H, Chuang SS, Munson P, Prada-Puentes C,Kim I, Du MQ, Bacon CM.
- xljin8
金主任列出CYCLIND1阳性的B细胞淋巴瘤，我也锦上添花，贴一篇T细胞淋巴瘤的文献，今天遇到1例皮肤cyclin D1阳性的原发性T细胞淋巴瘤。
J Invest Dermatol. 2006 Jun;126(6):1388-95.
Heterogeneous abnormalities of CCND1 and RB1 in primary cutaneous T-Cell lymphomas suggesting impaired cell cycle control in disease pathogenesis.
Mao X, Orchard G, Vonderheid EC, Nowell PC, Bagot M, Bensussan A, Russell-Jones R, Young BD, Whittaker SJ.
Source
Skin Tumour Unit, St John's Institute of Dermatology, St Thomas' Hospital, King's College, London, UK. mxmayo@yahoo.co.uk
Abstract
Upregulation of cyclin D1/B-cell leukemia/lymphoma 1 (CCND1/BCL1) is present in most mantle cell lymphomas with the t(11;14)(q13;q32) translocation. However, little is known about the abnormalities of CCND1 and its regulator RB1 in primary cutaneous T-cell lymphomas (CTCL). We analyzed CCND and RB status in CTCL using fluorescent in situ hybridization (FISH), immunohistochemistry (IHC), and Affymetrix expression microarray. FISH revealed loss of CCND1/BCL1 in five of nine Sézary syndrome (SS) cases but gain in two cases, and RB1 loss in four of seven SS cases. IHC showed absent CCND1/BCL1 expression in 18 of 30 SS, 10 of 23 mycosis fungoides (MF), and three of 10 primary cutaneous CD30+ anaplastic large-cell lymphoma (C-ALCL). Increased CCND1/BCL1 expression was seen in nine MF, seven C-ALCL, and six SS cases. Absent RB1 expression was detected in 8 of 12 MF and 7 of 9 SS cases, and raised RB1 expression in 7 of 8 C-ALCL. Affymetrix revealed increased gene expression of CCND2 in four of eight CTCL cases, CCND3 in three cases, and CDKN2C in two cases with a normal expression of CCND1 and RB1. These findings suggest heterogeneous abnormalities of CCND and RB in CTCL, in which dysregulated CCND and RB1 may lead to impaired cell cycle control.
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本帖最后由 肉肉卷 于 2012-04-25 12:12:56 编辑
昨天我也遇到了一例cyclinD1阳性的大B细胞淋巴瘤，因为细胞形态更倾向DLBCL，加上免疫组化bcl6+，CD5-，不支持套细胞淋巴瘤，母细胞变型，最后还是考虑出cyclinD1阳性的弥漫大B细胞淋巴瘤，这一例的ki-67也很高，70%左右，和楼主的病例接近，cyclinD1阳性的弥漫大B细胞淋巴瘤增殖指数高，是否也是其预后差的一个指针呢？另外，第一次遇到这样的病例，心里没底，想咨询专家还需要进一步做哪些工作？谢谢，再细读了一遍帖子后，心里又打鼓了，因为本例也是bcl2+
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引用 26 楼 肉肉卷 在 2012-04-25 11:37:00 的发言:
本帖最后由 肉肉卷 于 2012-04-25 12:12:56 编辑
昨天我也遇到了一例cyclinD1阳性的大B细胞淋巴瘤，因为细胞形态更倾向DLBCL，加上免疫组化bcl6+，CD5-，不支持套细胞淋巴瘤，母细胞变型，最后还是考虑出cyclinD1阳性的弥漫大B细胞淋巴瘤，这一例的ki-67也很高，70%左右，和楼主的病例接近，cyclinD1阳性的弥漫大B细胞淋巴瘤增殖指数高，是否也是其预后差的一个指针呢？另外，第一次遇到这样的病例，心里没底，想咨询专家还需要进一步做哪些工作？谢谢，再细读了一遍帖子后，心里又打鼓了，因为本例也是bcl2+
FISH是金标准。
对大B而言，
目前，没有证据显示cyclinD1有预后意义。
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