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肺穿组织：ck + ck5/6 + ck34BE12 + p63+ ck7+ cam5.2 + 。怎么都表达啊，怎么办？

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本帖最后由 xxmcwm 于 2011-12-15 21:37:51 编辑

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: 知我罪我，唯其春秋！

×参考诊断

基底样鳞状细胞癌

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1 楼发表于2011-12-15 18:05:08举报|引用
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肺穿组织：ck + ck5/6 + ck34BE12 + p63+ ，是鳞状细胞的表达； ck7+，是腺细胞的表达，如果您的病例HE确定是恶性，就要考虑粘液表皮样癌。

希望楼主上传HE和IHC图片，大家看图才好讨论！

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: 许春雷

xxmcwm 离线: 帖子：243; 粉蓝豆：36; 经验：332; 注册时间：2009-03-08; 加关注 | 发消息

2 楼发表于2011-12-15 21:35:13举报|引用
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免疫组化搞得我晕晕的

请教大家啦

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: 知我罪我，唯其春秋！

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3 楼发表于2011-12-15 22:31:28举报|引用
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这篇2010年的文献CK7可以表达于原发的鳞状细胞癌，呵呵，本例的有限心态学图片感觉好像还是倾向为鳞状细胞团块。你的发现是很有价值的。

J Cutan Pathol. 2010 Sep;37(9):966-72. Epub 2010 May 26.

CK7 expression in primary cutaneous squamous cell carcinoma.

Pulitzer M, Desman G, Busam KJ.

Source

Department of Pathology, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA. pulitzem@mskcc.org

Abstract

AIM:

To evaluate cytokeratin 7 (CK7) immunoreactivity in invasive primary cutaneous squamous cell carcinomas (SCCs).

METHODS:

Twenty-seven primary cutaneous SCCs from 25 patients were evaluated for tumor grade using hematoxylin and eosin-stained slides and for percentage and intensity of immunoreactivity for CK7. All cases exhibited features of SCC with an in situ component. No glandular or tubular differentiation was present. Staining intensity was graded on a scale of 0-3, with 0 indicating no reaction. Of immunoreactive cases, percentage of tumor staining and distribution of immunoreactivity was documented.

RESULTS:

Six of 27 SCCs (22%) exhibited immunostaining for CK7. Of those cases, three were poorly differentiated, exhibiting 2 to 3+ intensity in 5-15% of cells. Two were poorly differentiated, with 2 to 3+ intensity in 30-60% of cells. The remaining immunoreactive tumor was moderately differentiated, with 1+ intensity and 5% staining in an area of microinvasion.

CONCLUSION:

A subset of cutaneous SCCs, in particular, poorly differentiated tumors低分化肿瘤, may show focal-to-partial immunoreactivity for CK7可以出现部分或局灶的CK7表达. This is important to bear in mind when immunohistochemistry is used to distinguish SCC from simulants, such as porocarcinoma, or other adnexal carcinomas with squamous metaplasia在区分汗腺来源或是其他皮肤附属器来源肿瘤伴有的鳞状细胞化生时需要特别注意.

呵呵，楼主要是能收集一些其他部位如宫颈、食道、口腔、肺的癌做做CK7，看看表达的pattern，就可以发一篇sci的文章了，呵呵