这组抗体据说特别的实用。
Cluster analysis of immunohistochemical profiles delineates CK7, vimentin, S100A1 and C-kit (CD117) as an optimal
panel in the differential diagnosis of renal oncocytoma from its mimics
2011 Blackwell Publishing Ltd, Histopathology, 58, 169–179.
Aims: To develop an immunohistochemical strategy for
distinguishing renal oncocytoma (RO) from the eosinophilic
variant of chromophobe (ChRCC), and papillary
(PRCC) and clear cell (CRCC) renal cell carcinoma
containing eosinophilic cytoplasm in core biopsy specimens.
Methods and results: Cluster analysis was performed on
immunohistochemical data from 21 RO, 16 ChRCC, 16
CRCC and 20 PRCC patients. A panel of CK7, C-kit,
S100A1 and vimentin clustered into four groups.
Cluster A (94% ChRCC) expressed C-kit and CK7 and
lacked S100A1 and vimentin. Cluster B (95% RO)
expressed C-kit, S100A1, focal CK7 (single or small
clusters of cells) and lacked vimentin. Cluster Ccomprised a mixture of PRCC and CRCC with no
expression of C-kit or CK7 and variable S100A1 and
vimentin. PRCC with strong expression of CK7 clustered
into group D. A panel of S100A1 (positive) and
focal CK7 expression distinguished RO from ChRCC
with 91% sensitivity and 93% specificity. A panel of
vimentin (negative) and C-kit (positive) distinguished
RO from CRCC with 83% sensitivity and 86% specificity
and RO from PRCC with 79% sensitivity and 88%
specificity.
Conclusions: Hierarchical cluster analysis is an effective
approach to analyse high-volume immunohistochemical
data to generate an optimal panel in the differential
diagnosis of oncocytoma from its mimics.