On immunohistochemical analysis, both in paraffin wax embedded and frozen sections, the antibodies against CD30 produce different types of positivity: membrane bound or dot-like in the Golgi area (corresponding to the accumulation of the 90 kDa proteic precursor) and diffuse，The first two patterns are exclusive to lymphoid elements  with the exception of embryonic carcinoma ，whereas the diffuse pattern can occur in a variety of malignant tumours other than lymphomas, including pancreatic carcinoma, nasopharyngeal undifferentiated carcinoma, and malignant melanoma.

 Schwarting R, Gerdes J, Durkop H, et al. Ber-H2: a new anti-Ki-1(CD-30) monoclonal antibody directed at a formol resistant epitope.Immunohistochemical and immunobiochemical characterization.Blood1989;74:1678–89