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- 请问侯军老师有关骨髓普通石蜡切片的制片问题
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本帖最后由 于 2010-07-28 10:55:00 编辑
切片
Microtoming
1、骨髓切片应在3-4微米厚1.Cut bone marrow cores at 3-4 microns.
2。放3个切面在一张片子上，最好多切几张片子，以备特殊染色用2. Place 3 levels on one slide. Cut an extra slide of the last level and set aside
on the back of the water bath in case a special stain is requested.
Note:
过度脱钙将导致无法分辨的组织和细胞的细节。 小于一个小时的脱钙时间常常是不够。Over decalcification will result in poor or indifferent histological detail and
staining characteristics. Less than one hour for slow decal is usually not
sufficient.
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本帖最后由 于 2010-08-23 07:02:00 编辑
翻译侯老师的内容：
下面是我准备的骨髓脱钙的程序，但是，我不知道怎样配制脱钙液。我们通常从FISHer公司买这些产品。我们在常规诊断时，除HE染色外，我们还准备Giemsa 染色。（I prepared the procedure for bone marrow decalcification. But, I do not know how to prepare the decal solution. We usually buy these products from Fisher. Other than the H&E slide, we routinely prepare Giemsa slide for diagnosis. Good Luck.）
(Thank you in advance for translating this procedure)
骨髓活检（BONE MARROW BIOPSIES）：
骨髓活检组织的固定
（Bone Marrow Core Fixation）
1、组织要在福尔马林中至少固定一小时
1.
Immediately fix in Formailn for a minimum of one hour.
2、如果标本在工作日结束前2小时收到，固定过夜
2.
Fix overnight if brought in later than 2 hours before end of the work day.
第二天上午脱钙 Decalcify first thing in the morning.
骨髓活检组织脱钙
Bone Marrow Core Decalcification
1、把固定好的骨髓组织在一个小塑料 Cassette 中。
1.
Place fixed marrow core in a plastic cassette.
在supper decal 1（慢脱钙）液中脱钙：（ 缓和脱钙，） 脱钙1小时
Decalcify in Super Decal I (Slow Decal): Delicate Decal, Decalcify the Decal Block
Solution (Stat Decal) or Deli-Cal Block Solution for 1 hour.
检查组织是否变软
Test for decalcification by gently
checking for pliability
当脱钙完成后，用流水冲洗5分钟
When ready, rinse in running water
for 5 minutes.
2、如果应用快速脱钙液（super Decal II：强脱钙液）：骨髓组织脱钙15-30分钟
2.
If use the fast decal solution (Super Decal II: Heavy Duty Decal) decalcifying
solution, decalcify the bone marrow core for 15-30 minutes.
检查是否变软
Test for decalcification by gently
checking for pliability
流水冲洗大概5分钟
Rinse in running tap water for
approximately 5 minutes.
千万不要将组织留在脱钙液中过夜。
用计时器帮助记时。
我们用一个震荡器 （ Shaker ) 帮助脱钙。
Do not leave overnight. Use
timer. We use a shaker to facilitate the decal procedure.
