请问侯军老师有关骨髓普通石蜡切片的制片问题

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楼主发表于 2010-07-15 23:19|举报|关注(2)
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医院里血液科很可以，经常碰到骨髓穿刺标本，但由于脱钙或其他问题，切片很难看，基本的细胞成分有时都分不清。上次听侯军老师讲课提到快速脱钙液对骨髓的处理可以保持良好的形态，所以想请问侯军老师那个快速脱钙液是如何配制的，脱钙应该是多久，常温还是烘箱或微波炉处理，在整个制片中应该注意什么？诊断时除看HE染色外还要看涂片或其他染色的石蜡切片吗？谢谢。

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1 楼发表于2010-07-18 07:44:00举报|引用
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I prepared the procedure for bone marrow decalcification. But, I do not know how to prepare the decal solution. We usually buy these products from Fisher. Other than the H&E slide, we routinely prepare Giemsa slide for diagnosis. Good Luck.
(Thank you in advance for translating this procedure)

BONE MARROW BIOPSIES

Bone Marrow Core Fixation

1. Immediately fix in Formailn for a minimum of one hour.

2. Fix overnight if brought in later than 2 hours before end of the work day.

Decalcify first thing in the morning.

Bone Marrow Core Decalcification

1. Place fixed marrow core in a plastic cassette.

Decalcify in Super Decal I (Slow Decal): Delicate Decal, Decalcify the Decal Block Solution (Stat Decal) or Deli-Cal Block Solution for 1 hour.

Test for decalcification by gently checking for pliability

When ready, rinse in running water for 5 minutes.

2. If use the fast decal solution (Super Decal II: Heavy Duty Decal) decalcifying solution, decalcify the bone marrow core for 15-30 minutes.

Test for decalcification by gently checking for pliability

Rinse in running tap water for approximately 20 minutes.

Do not leave overnight. Use timer. We use a shaker to facilitate the decal procedure.

Processing

1. Process as usual

Microtoming

1. Cut bone marrow cores at 3-4 microns.

2. Place 3 levels on one slide. Cut an extra slide of the last level and set aside on the back of the water bath in case a special stain is requested.

Note:

Over decalcification will result in poor or indifferent histological detail and staining characteristics. Less than one hour for slow decal is usually not sufficient.

Please test the procedure adequately before use it for patient specimen.

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2 楼发表于2010-07-18 19:39:00举报|引用
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Thank you very much.

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3 楼发表于2010-07-21 10:28:00举报|引用
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请gwls0008 朋友或海燕在有时间时翻译一下好吗？

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njlihua 离线: 帖子：113; 粉蓝豆：14; 经验：158; 注册时间：2007-03-31; 加关注 | 发消息

4 楼发表于2010-07-21 23:06:00举报|引用
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本帖最后由于 2010-08-23 07:02:00 编辑

翻译侯老师的内容：

下面是我准备的骨髓脱钙的程序，但是，我不知道怎样配制脱钙液。我们通常从FISHer公司买这些产品。我们在常规诊断时，除HE染色外，我们还准备Giemsa 染色。（I prepared the procedure for bone marrow decalcification. But, I do not know how to prepare the decal solution. We usually buy these products from Fisher. Other than the H&E slide, we routinely prepare Giemsa slide for diagnosis. Good Luck.）
(Thank you in advance for translating this procedure)

骨髓活检（BONE MARROW BIOPSIES）：

骨髓活检组织的固定

（Bone Marrow Core Fixation）

1、组织要在福尔马林中至少固定一小时

1.
Immediately fix in Formailn for a minimum of one hour.

2、如果标本在工作日结束前2小时收到，固定过夜

2.
Fix overnight if brought in later than 2 hours before end of the work day.

第二天上午脱钙

Decalcify first thing in the morning.

骨髓活检组织脱钙

Bone Marrow Core Decalcification

1、把固定好的骨髓组织在一个小塑料 Cassette 中。

1.
Place fixed marrow core in a plastic cassette.

在supper decal 1（慢脱钙）液中脱钙：（缓和脱钙，）脱钙1小时

Decalcify in Super Decal I (Slow Decal): Delicate Decal, Decalcify the Decal Block
Solution (Stat Decal) or Deli-Cal Block Solution for 1 hour.

检查组织是否变软

Test for decalcification by gently
checking for pliability

当脱钙完成后，用流水冲洗5分钟

When ready, rinse in running water
for 5 minutes.

2、如果应用快速脱钙液（super Decal II：强脱钙液）：骨髓组织脱钙15-30分钟

2.
If use the fast decal solution (Super Decal II: Heavy Duty Decal) decalcifying
solution, decalcify the bone marrow core for 15-30 minutes.

检查是否变软

Test for decalcification by gently
checking for pliability

流水冲洗大概5分钟

Rinse in running tap water for
approximately 5 minutes.

千万不要将组织留在脱钙液中过夜。

用计时器帮助记时。

我们用一个震荡器（ Shaker ) 帮助脱钙。

Do not leave overnight. Use
timer. We use a shaker to facilitate the decal procedure.

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5 楼发表于2010-07-21 23:13:00举报|引用
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本帖最后由于 2010-07-28 10:55:00 编辑 切片

Microtoming

1、骨髓切片应在3-4微米厚

1.Cut bone marrow cores at 3-4 microns.

2。放3个切面在一张片子上，最好多切几张片子，以备特殊染色用

2. Place 3 levels on one slide. Cut an extra slide of the last level and set aside
on the back of the water bath in case a special stain is requested.

注意：

Note:

过度脱钙将导致无法分辨的组织和细胞的细节。小于一个小时的脱钙时间常常是不够。

Over decalcification will result in poor or indifferent histological detail and
staining characteristics. Less than one hour for slow decal is usually not
sufficient.

在用于病人标本之前请充分检测每个细节。Pleasetest the procedure adequately before use it for patient specimen.

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6 楼发表于2010-07-28 11:16:00举报|引用
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谢谢njlihua 的翻译。我作了一点修改。希望对大家有帮助。

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7 楼发表于2010-08-22 10:06:00举报|引用
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谢谢喉军老师，谢谢njlihua 的翻译，辛苦了。

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: 我喜欢春天，更喜欢华夏病理网

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8 楼发表于2010-08-28 07:34:00举报|引用
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请问supper decal 1和supper decal 2 怎样配置，谢谢！

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9 楼发表于2010-09-01 11:07:00举报|引用
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I do not know how to prepare these solutions. We buy these from commercial company. You can try some decal solutions which are available in China.
Hou Jun

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