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- 大网膜肿瘤（附免疫组化），请发表意见！
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wangdingding 离线
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本帖最后由 于 2010-02-17 10:04:00 编辑
IHC标记结果：
1）阳性表达：Calretinin -/+; Calponin +; SMA-/+; WT-1+/-;CD99+;Bcl-2+;NSE+/-;Leu-7+/-; Ki-67 60%+。
2）不表达：CD34；CD117；CEA；Mesothelium；LCA；CK pan；EMA；CK7；CK20；CK5/6；CK-LMW；CK-HMW-；Actin；Desmin；MyoD1；Crg；S-100；Syn；D2-40；PLAP；CD10；CD68；CD43；MPO。
- xljin8
| 以下是引用海上明月在2010-1-28 2:44:00的发言：
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- 当你有选择的时候，不是选择正确的，而是选择不让你后悔的!
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本帖最后由 于 2010-01-28 02:31:00 编辑
Cancer Sci. 2003 Mar;94(3):271-6.
Overexpression of the Wilms' tumor gene WT1 in human bone and soft-tissue sarcomas.
Ueda T, Oji Y, Naka N, Nakano Y, Takahashi E, Koga S, Asada M, Ikeba A, Nakatsuka S, Abeno S, Hosen N, Tomita Y, Aozasa K, Tamai N, Myoui A, Yoshikawa H, Sugiyama H.
Department of Orthopaedic Surgery, Osaka University Graduate School of Medicine, Suita, Osaka.
The expression levels of the Wilms' tumor gene WT1 were examined in 36 cases of various types of human bone and soft-tissue sarcomas using quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR). They included 12 malignant fibrous histiocytomas (MFH), 3 malignant peripheral nerve sheath tumors (MPNST), 6 synovial sarcomas (SyS), 4 myxoid liposarcomas (MyLS), one angiosarcoma (AGS), one clear cell sarcoma (CCS), and 9 osteosarcomas (OS). Eleven (92%) of 12 MFH, 2 (67%) of 3 MPNST, all (100%) of 6 SyS, 2 (50%) of 4 MyLS, one AGS, one CCS, and 5 (56%) of 9 OS cases overexpressed WT1 in the range of 1.4 x 10(-3)-3.9 x 10(-1) levels (WT1 expression level in K562 leukemic cells was defined as 1.0). Thus, 28 (78%) out of 36 various types of human bone and soft-tissue sarcomas overexpressed the WT1 gene. Immunohistochemical analysis showed positive staining for WT1 protein in all of 4 cases (one case each of MFH, MyLS, AGS and OS) with WT1 gene overexpression detected by RT-PCR analysis, demonstrating clearly that WT1 was expressed at the protein level in various types of human bone and soft-tissue sarcomas. The direct sequencing analysis of the WT1 genomic DNA showed no mutations in any of 10 exons of the WT1 gene in 8 different sarcoma samples (3 MFH, one SyS, one MyLS, one AGS, and 2 OS). The present study demonstrates that various types of human bone and soft-tissue sarcomas frequently overexpress the wild-type WT1 gene, suggesting an important role of the wild-type WT1 gene in tumorigenesis of various types of human bone and soft-tissue sarcomas.
- 王军臣
