楼主 发表于 2007-05-05 22:07|举报|关注(0)

浏览排序[ 顺序 逆序 楼主 支持 精彩 ]  快捷回复

Paul B Fisher

An obstacle to effective gene-based cancer therapies is the limited number of cancer-specific growth suppressing and apoptosis-inducing genes. Using a differentiation induction subtraction hybridization (DISH) approach with human melanoma cells, melanoma differentiation associated (mda) genes were isolated that display elevated expression as a function of irreversible growth arrest, cancer reversion and terminal differentiation. This screening paradigm resulted in the cloning of mda-7 in the context of terminal differentiation of human melanoma cells. Based on its structure, chromosomal location, sequence homology and cytokine-like properties, mda-7 has now been renamed IL-24 and classified as a member of the expanding IL-10 cytokine gene family. Expression of mda-7/IL-24 inversely correlates with melanoma progression and administration of mda-7/IL-24 by means of a replication incompetent adenovirus, Ad.mda-7, results in growth suppression and apoptosis in melanoma cells as well as in a broad-spectrum of additional cancer cell types. In contrast, Ad.mda-7 does not elicit deleterious effects in normal cells, including those of epithelial, fibroblast, astrocyte, melanocyte or endothelial origin. Based on these distinctive properties and anti-tumor and anti-angiogenic activities in human tumor xenograft animal models, mda-7/IL-24 has now entered the clinical arena. A Phase I/II clinical trial in patients with advanced carcinomas involving intratumoral administration of mda-7/IL-24 [using a replication incompetent adenovirus; ING241 (Ad.mda-7)] has documented that this gene is safe and well tolerated by patients and a single virus injection elicits apoptosis in a majority of the tumor. Current data suggests that mda-7/IL-24 may function as a dual-acting cytokine in which its normal physiological functions may be related to specific aspects of the immune system and over-expre

0

用心做事、真情做人！

添加参考诊断

1 楼    发表于2007-05-08 09:07:00举报|引用

返回顶部 | 快捷回复

 要是配上翻译和图片就好了，^_^。

0

吾表兄，年四十余。始从文，连考三年而不中。遂习武，练武场上发一矢，中鼓吏，逐之出。改学医，自撰一良方，服之，卒。

2 楼    发表于2007-05-13 01:39:00举报|引用

返回顶部 | 快捷回复

王老师的建议很好,我简单翻译一下.

基因治疗的困难之一就是缺乏特异性的肿瘤生长抑制和凋亡的基因.对人的黑色素瘤细胞应用分化诱导差异杂交技术,分离发现了黑色素瘤细胞分化相关基因(MDA家族),

0

用心做事、真情做人！